Hotspot

trnS-GCU-trnG-UCC

LSC · 8952-9836 · 885 bp

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Pi 0.0126
MarkerSeek score 62.10
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
885
variable_sites
11
pi
0.0126
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TGGAATCTAAGCCGAGGGAT 50.0 50.0 58.0 57.9 994-995 bp 1.0 80.8
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TGGAATCTAAGCCGAGGGAT 50.0 50.0 58.0 57.9 993-994 bp 1.0 80.8
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA TTGGAATCTAAGCCGAGGGA 50.0 50.0 58.0 58.1 995-996 bp 1.0 80.6
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC TTGGAATCTAAGCCGAGGGA 50.0 50.0 58.0 58.1 994-995 bp 1.0 80.6
trnS-GCU-trnG-UCC_p5 ATCATCCATCAACGGAACCG TGGAATCTAAGCCGAGGGAT 50.0 50.0 58.0 57.9 1065-1066 bp 1.0 80.4