Hotspot

ycf1

SSC · 128081-133678 · 5598 bp

Back to Ochagavia

Pi 0.00841

MarkerSeek score 48.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5598
variable_sites: 47
pi: 0.00841
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGAAATTGATCCTGTGAATGGT	AGAACGAATCACAAAAGACAAGA	39.1	34.8	58.3	56.7	204 bp	1.0	42.4
ycf1_p2	TGGAAATTGATCCTGTGAATGG	AGAACGAATCACAAAAGACAAGA	40.9	34.8	56.7	56.7	204 bp	1.0	42.4
ycf1_p3	GGAAATTGATCCTGTGAATGGT	AGAACGAATCACAAAAGACAAGA	40.9	34.8	56.7	56.7	203 bp	1.0	42.4
ycf1_p4	TGGAAATTGATCCTGTGAATGGT	AGAACGAATCACAAAAGACAAGAA	39.1	33.3	58.3	57.3	204 bp	1.0	42.4
ycf1_p5	TTGGAAATTGATCCTGTGAATGG	AGAACGAATCACAAAAGACAAGA	39.1	34.8	57.3	56.7	205 bp	1.0	42.3