Hotspot

atpF

LSC · 12786-14174 · 1389 bp

Back to Ochagavia

Pi 0.00792

MarkerSeek score 48.80

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1389
variable_sites: 11
pi: 0.00792
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGCTCCTGGAAAGTAGTCAC	GGACTGCTTTGAGGATGAGG	55.0	55.0	58.0	58.0	2410-2416 bp	1.0	80.2
atpF_p2	CGCTCCTGGAAAGTAGTCAC	ATTTCTGCCGCTTCCGTTAT	55.0	45.0	58.0	58.0	2792-2798 bp	1.0	80.2
atpF_p3	CGCTCCTGGAAAGTAGTCAC	TTGCCGTTTCCTTCTTCGAA	55.0	45.0	58.0	58.0	2481-2487 bp	1.0	80.1
atpF_p4	CGCTCCTGGAAAGTAGTCAC	GTTGTGGCATTAGCGCTTTT	55.0	45.0	58.0	57.9	2600-2606 bp	1.0	80.0
atpF_p5	CGCTCCTGGAAAGTAGTCAC	TGAGACTCCAGGAAGGACTG	55.0	55.0	58.0	58.1	2424-2430 bp	1.0	79.9