Hotspot

ndhI-ndhA

SSC · 123894-123981 · 88 bp

Back to Ochagavia

Pi 0.0227

MarkerSeek score 65.60

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 88
variable_sites: 2
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	ACCAATGTACCTTGCAGCTC	ACCTAGGATGAGAATGGACCA	50.0	47.6	58.2	57.6	266 bp	1.0	67.8
ndhI-ndhA_p2	GACCAATGTACCTTGCAGCT	ACCTAGGATGAGAATGGACCA	50.0	47.6	58.2	57.6	267 bp	1.0	67.8
ndhI-ndhA_p3	ACCTTGCAGCTCGTATTGTT	ACCTAGGATGAGAATGGACCA	45.0	47.6	57.8	57.6	258 bp	1.0	67.8
ndhI-ndhA_p4	TGACCAATGTACCTTGCAGC	ACCTAGGATGAGAATGGACCA	50.0	47.6	58.5	57.6	268 bp	1.0	65.5
ndhI-ndhA_p5	CAATGTACCTTGCAGCTCGT	ACCTAGGATGAGAATGGACCA	50.0	47.6	58.6	57.6	264 bp	1.0	64.8