Hotspot

petB-petD

LSC · 79734-80622 · 889 bp

Back to Ochagavia

Pi 0.00904

MarkerSeek score 64.40

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 889
variable_sites: 8
pi: 0.00904
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	AAGCAAGGTATTTCCGGTCC	ATGTCCCATCCCTTTAGCCA	50.0	50.0	57.9	58.4	1026-1030 bp	1.0	76.8
petB-petD_p2	GCAAGGTATTTCCGGTCCTT	ATGTCCCATCCCTTTAGCCA	50.0	50.0	57.9	58.4	1024-1028 bp	1.0	76.8
petB-petD_p3	AAGCAAGGTATTTCCGGTCC	TGTCCCATCCCTTTAGCCAA	50.0	50.0	57.9	58.6	1025-1029 bp	1.0	75.0
petB-petD_p4	GCAAGGTATTTCCGGTCCTT	TGTCCCATCCCTTTAGCCAA	50.0	50.0	57.9	58.6	1023-1027 bp	1.0	75.0
petB-petD_p5	GCAAGGTATTTCCGGTCCTT	GTCCCATCCCTTTAGCCAAT	50.0	50.0	57.9	57.2	1022-1026 bp	1.0	73.9