Hotspot

accD-psaI

LSC · 61110-62031 · 922 bp

Back to Ochagavia

Pi 0.00871

MarkerSeek score 64.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 922
variable_sites: 8
pi: 0.00871
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTCCATGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1036-1070 bp	1.0	78.8
accD-psaI_p2	GCTCCATGGTTTCTTTCCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1035-1069 bp	1.0	78.8
accD-psaI_p3	AGCTCCATGGTTTCTTTCCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	1042-1076 bp	1.0	78.2
accD-psaI_p4	GCTCCATGGTTTCTTTCCCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	1041-1075 bp	1.0	78.2
accD-psaI_p5	GACCCAATCGTACCACGTAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.3	58.0	1088-1122 bp	1.0	75.4