Hotspot

atpB-rbcL

LSC · 56728-57523 · 796 bp

Back to Ochagavia

Pi 0.0138

MarkerSeek score 66.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 796
variable_sites: 11
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGGGAAAAACGACATCCAGT	ACACCAGCTTTAAATCCAGCA	50.0	42.9	58.1	58.1	955-958 bp	1.0	71.2
atpB-rbcL_p2	GGGGAAAAACGACATCCAGT	ACCAGCTTTAAATCCAGCACT	50.0	42.9	58.1	57.8	953-956 bp	1.0	70.9
atpB-rbcL_p3	TGGGCCAATAATTTGAGCGA	ACACCAGCTTTAAATCCAGCA	45.0	42.9	57.9	58.1	933-936 bp	1.0	70.9
atpB-rbcL_p4	TGAGCGATACACCCTAGGTT	ACACCAGCTTTAAATCCAGCA	50.0	42.9	57.8	58.1	920-923 bp	1.0	70.7
atpB-rbcL_p5	TTGAGCGATACACCCTAGGT	ACACCAGCTTTAAATCCAGCA	50.0	42.9	57.8	58.1	921-924 bp	1.0	70.7