Hotspot

trnF-ndhJ

LSC · 50289-51051 · 763 bp

Back to Ochagavia

Pi 0.0162

MarkerSeek score 58.20

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 11
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCATTGA	50.0	31.8	58.1	53.8	736-819 bp	1.0	45.1
trnF-ndhJ_p2	AATCCTCGTGTCACCAGTTC	TGAAATACAAGATGCTCATTGA	50.0	31.8	57.5	53.8	739-822 bp	1.0	45.1
trnF-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATGAAATACAAGATGCTCATTGA	50.0	30.4	58.1	54.4	737-820 bp	1.0	45.1
trnF-ndhJ_p4	TGGTAGAGCAGAGGACTGAA	TGAAATACAAGATGCTCATTGA	50.0	31.8	57.4	53.8	759-842 bp	1.0	45.1
trnF-ndhJ_p5	TTGGTAGAGCAGAGGACTGA	TGAAATACAAGATGCTCATTGA	50.0	31.8	57.4	53.8	760-843 bp	1.0	45.1