Hotspot

psbC-trnS

LSC · 37733-37869 · 137 bp

Back to Ochagavia

Pi 0.0511

MarkerSeek score 73.40

Primer available yes

Label psbC-trnS

Feature Metadata

label_name: psbC-trnS
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 7
pi: 0.0511
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS_p1	GCTGCAGGGTTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.1	58.1	283 bp	1.0	80.5
psbC-trnS_p2	GGCTGCAGGGTTTGAAAAAG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.1	58.1	284 bp	1.0	80.5
psbC-trnS_p3	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	244 bp	1.0	80.5
psbC-trnS_p4	GCTGCAGGGTTTGAAAAAGG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.1	58.1	294 bp	1.0	79.8
psbC-trnS_p5	GGCTGCAGGGTTTGAAAAAG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.1	58.1	295 bp	1.0	79.8