Hotspot

trnS-trnR

LSC · 8705-10997 · 2293 bp

Back to Ochagavia

Pi 0.0133

MarkerSeek score 61.70

Primer available yes

Label trnS-trnR

Feature Metadata

label_name: trnS-trnR
feature_type: igs
region: LSC
length_bp: 2293
variable_sites: 21
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnR_p1	GAGAAAGCGGAAAGAGAGGG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.0	56.9	1753-2424 bp	1.0	68.3
trnS-trnR_p2	ATTAGCAATCCGCCGCTTTA	AGAAGACCTCTGTCCTATCCA	45.0	47.6	58.0	56.9	1698-2369 bp	1.0	68.1
trnS-trnR_p3	GAGAAAGCGGAAAGAGAGGG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.0	57.9	1759-2430 bp	1.0	67.6
trnS-trnR_p4	ATTAGCAATCCGCCGCTTTA	AGGTTTAGAAGACCTCTGTCCT	45.0	45.5	58.0	57.9	1704-2375 bp	1.0	67.4
trnS-trnR_p5	AGAAAGCGGAAAGAGAGGGA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.4	56.9	1752-2423 bp	1.0	65.6