Hotspot

rps16-trnQ

LSC · 5823-7347 · 1525 bp

Back to Ochagavia

Pi 0.0163

MarkerSeek score 71.50

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1525
variable_sites: 24
pi: 0.0163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	CAGCCGATCATGTCCTTCAA	GAAATCAAAATGGGGCGTGG	50.0	50.0	58.0	58.0	1620-1675 bp	1.0	83.5
rps16-trnQ_p2	GCCGATCATGTCCTTCAAGT	GAAATCAAAATGGGGCGTGG	50.0	50.0	58.0	58.0	1618-1673 bp	1.0	83.5
rps16-trnQ_p3	AATCCACAGCCGATCATGTC	GAAATCAAAATGGGGCGTGG	50.0	50.0	58.0	58.0	1626-1681 bp	1.0	83.4
rps16-trnQ_p4	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1562-1617 bp	1.0	83.1
rps16-trnQ_p5	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1560-1615 bp	1.0	83.1