Hotspot

trnK-rps16

LSC · 4048-4742 · 695 bp

Back to Ochagavia

Pi 0.0144

MarkerSeek score 62.90

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 10
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	804 bp	1.0	78.1
trnK-rps16_p2	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	803 bp	1.0	78.1
trnK-rps16_p3	TACCGTTGAGTTAGCAACCC	GGTGCTCAACCTACAGGAAC	50.0	55.0	57.5	58.2	790 bp	1.0	75.8
trnK-rps16_p4	AGTCGCACTTAAAAGCCGAG	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.6	58.2	815 bp	1.0	74.9
trnK-rps16_p5	TCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAGGAAC	45.0	55.0	57.3	58.2	813 bp	1.0	73.6