Hotspot

rpoC1

LSC · 20234-23036 · 2803 bp

Back to Oberonia

Pi 0.0107

MarkerSeek score 47.90

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2803
variable_sites: 45
pi: 0.0107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	AGTATAGCATGAGTTAGCTAGC	TCCAGATGAATAGGAAGGAAGT	40.9	40.9	55.0	56.0	2877-2883 bp	0.666667	27.4
rpoC1_p2	AGTATAGCATGAGTTAGCTAGC	AGGAAGGAAGTTTGATCTCAATGA	40.9	37.5	55.0	57.9	2866-2872 bp	0.666667	27.4
rpoC1_p3	AGTATAGCATGAGTTAGCTAGC	TCCAGATGAATAGGAAGGAAGTT	40.9	39.1	55.0	56.7	2877-2883 bp	0.666667	27.4
rpoC1_p4	AGTATAGCATGAGTTAGCTAGC	TTCCAGATGAATAGGAAGGAAGT	40.9	39.1	55.0	56.7	2878-2884 bp	0.666667	27.4
rpoC1_p5	AGTATAGCATGAGTTAGCTAGC	AGGAAGGAAGTTTGATCTCA	40.9	40.0	55.0	53.5	2866-2872 bp	0.666667	27.4