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clpP-psbB

LSC · 71357-71995 · 639 bp

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Pi 0.0735

MarkerSeek score 81.40

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 68
pi: 0.0735
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CGGATCAAGAACAGGGTCAG	50.0	55.0	58.0	58.0	877-918 bp	1.0	87.3
clpP-psbB_p2	CGCACTATACGTCAACCCAA	ACTCCAAATGTCCGGATTCG	50.0	50.0	58.0	58.0	991-1032 bp	1.0	86.5
clpP-psbB_p3	CGCACTATACGTCAACCCAA	CTCCAAATGTCCGGATTCGT	50.0	50.0	58.0	58.0	990-1031 bp	1.0	86.5
clpP-psbB_p4	CGCACTATACGTCAACCCAA	ACACCCATATTCCAGGACCA	50.0	50.0	58.0	58.0	1220-1261 bp	1.0	85.9
clpP-psbB_p5	CGCACTATACGTCAACCCAA	TAGACCCGCTAATATGCCCA	50.0	50.0	58.0	58.0	1357-1398 bp	1.0	85.5