Hotspot

psaI-ycf4

LSC · 58300-58724 · 425 bp

Back to Oberonia

Pi 0.0566

MarkerSeek score 79.50

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 425
variable_sites: 33
pi: 0.0566
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCAATCGCAATGGCTTCTTT	CAGTACGGGTCAAGGGAATG	45.0	55.0	58.0	58.0	929-951 bp	1.0	86.1
psaI-ycf4_p2	GCAATCGCAATGGCTTCTTT	ATCAGTACGGGTCAAGGGAA	45.0	50.0	58.0	58.1	931-953 bp	1.0	85.9
psaI-ycf4_p3	GCAATCGCAATGGCTTCTTT	TCAGTACGGGTCAAGGGAAT	45.0	50.0	58.0	58.1	930-952 bp	1.0	85.9
psaI-ycf4_p4	GCAATCGCAATGGCTTCTTT	CTCCTTGGCCCCTTATTTCC	45.0	55.0	58.0	57.9	908-930 bp	1.0	85.8
psaI-ycf4_p5	GCAATCGCAATGGCTTCTTT	AGGTACGCGCAAGAAATAGG	45.0	50.0	58.0	58.1	997-1019 bp	1.0	85.4