Hotspot

atpB-rbcL

LSC · 52842-53827 · 986 bp

Back to Oberonia

Pi 0.0519

MarkerSeek score 79.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 986
variable_sites: 68
pi: 0.0519
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AGGGGGCTAAAAGATCCACT	GTCCACACAGTTGTCCATGT	50.0	50.0	58.0	58.0	1599-1703 bp	1.0	87.2
atpB-rbcL_p2	TTTTTCCTCCACGGCGATAG	GTCCACACAGTTGTCCATGT	50.0	50.0	58.0	58.0	1617-1721 bp	1.0	87.2
atpB-rbcL_p3	AGGGGGCTAAAAGATCCACT	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	2042-2146 bp	1.0	86.9
atpB-rbcL_p4	ATCTTGCCCGGTGGAAAAAT	AGAAACGATCTCTCCAACGC	45.0	50.0	58.1	58.0	1677-1781 bp	1.0	86.9
atpB-rbcL_p5	TTTTTCCTCCACGGCGATAG	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	2060-2164 bp	1.0	86.8