Hotspot

trnC-GCA-petN

LSC · 27691-28355 · 665 bp

Back to Oberonia

Pi 0.0331

MarkerSeek score 77.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 32
pi: 0.0331
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTGGATGAAATTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	760-788 bp	1.0	84.9
trnC-GCA-petN_p2	CCAGTTCGAATCTGGGTGTC	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.3	58.2	699-727 bp	1.0	84.0
trnC-GCA-petN_p3	GATGAAATTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	756-784 bp	1.0	82.2
trnC-GCA-petN_p4	GGATGAAATTTGGCGGCATG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	757-785 bp	1.0	82.2
trnC-GCA-petN_p5	TTTGGATGAAATTTGGCGGC	CCCACTCCTTCCCCATACTA	45.0	55.0	57.9	57.5	817-845 bp	1.0	81.7