Hotspot

rpoC2

LSC · 16930-21096 · 4167 bp

Pi 0.00048

MarkerSeek score 53.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4167
variable_sites: 3
pi: 0.00048
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CCCGATGGGTCATTCCTTTT	GCAAACCAATTCGGGGAATT	50.0	45.0	57.9	57.2	214 bp	1.0	73.1
rpoC2_p2	CCCGATGGGTCATTCCTTTT	GCAAACCAATTCGGGGAATTT	50.0	42.9	57.9	57.9	214 bp	1.0	70.8
rpoC2_p3	CCCGATGGGTCATTCCTTTT	GGCAAACCAATTCGGGGAAT	50.0	50.0	57.9	59.1	215 bp	1.0	70.4
rpoC2_p4	TGCGATCTGACCCATTTTCA	GCAAACCAATTCGGGGAATT	45.0	45.0	57.5	57.2	249 bp	1.0	70.3
rpoC2_p5	GGTGCGATCTGACCCATTTT	GCAAACCAATTCGGGGAATT	50.0	45.0	58.5	57.2	251 bp	1.0	69.9