Hotspot

rpoC1

LSC · 21247-24053 · 2807 bp

Back to Nyssa

Pi 0.00095
MarkerSeek score 44.10
Primer available yes
Label rpoC1

Feature Metadata

label_name
rpoC1
feature_type
gene
region
LSC
length_bp
2807
variable_sites
4
pi
0.00095
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1_p1 TGAACCGGAGAGACCTGAAT TTGCTCGTTCGAGAACTACG 50.0 50.0 58.1 58.0 2974 bp 1.0 79.3
rpoC1_p2 TGAACCGGAGAGACCTGAAT ACGATCTTTGGCTCTGGAAC 50.0 50.0 58.1 57.9 2957 bp 1.0 78.8
rpoC1_p3 AGACCTGAATTCCCACTGGT TTGCTCGTTCGAGAACTACG 50.0 50.0 58.3 58.0 2964 bp 1.0 77.8
rpoC1_p4 TTGAACCGGAGAGACCTGAA TTGCTCGTTCGAGAACTACG 50.0 50.0 58.3 58.0 2975 bp 1.0 77.5
rpoC1_p5 AGACCTGAATTCCCACTGGT ACGATCTTTGGCTCTGGAAC 50.0 50.0 58.3 57.9 2947 bp 1.0 77.3