Hotspot

psbD

LSC · 34025-35086 · 1062 bp

Back to Nyssa

Pi 0.00126
MarkerSeek score 47.10
Primer available yes
Label psbD

Feature Metadata

label_name
psbD
feature_type
gene
region
LSC
length_bp
1062
variable_sites
2
pi
0.00126
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD_p1 TGAGGTGTTCGGAAATGGTC GTTTCTTGATCACGACCGGA 50.0 50.0 57.8 57.9 1143 bp 1.0 78.1
psbD_p2 TGATCGAATCTTCAGACGCC GTTTCTTGATCACGACCGGA 50.0 50.0 57.8 57.9 1175 bp 1.0 77.9
psbD_p3 TGAGGTGTTCGGAAATGGTC ACCGGTGGTTTCTTGATCAC 50.0 50.0 57.8 58.1 1150 bp 1.0 77.8
psbD_p4 GCAGTGCGCAAGAAATCAAA GTTTCTTGATCACGACCGGA 45.0 50.0 58.2 57.9 1206 bp 1.0 77.7
psbD_p5 TGATCGAATCTTCAGACGCC ACCGGTGGTTTCTTGATCAC 50.0 50.0 57.8 58.1 1182 bp 1.0 77.6