Hotspot

ndhF

SSC · 112219-114453 · 2235 bp

Pi 0.000597

MarkerSeek score 57.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 2
pi: 0.000597
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGGATGGGAATCAAGAAAATTCG	GAGGTAAGATCCGGCGAATC	39.1	55.0	57.4	57.9	2364 bp	1.0	50.9
ndhF_p2	TGGATGGGAATCAAGAAAATTCG	CGAAAGAGGTAAGATCCGGC	39.1	55.0	57.4	58.1	2369 bp	1.0	50.3
ndhF_p3	TGGATGGGAATCAAGAAAATTCG	GAAAGAGGTAAGATCCGGCG	39.1	55.0	57.4	58.1	2368 bp	1.0	50.3
ndhF_p4	TGGATGGGAATCAAGAAAATTCG	AGAGGTAAGATCCGGCGAAT	39.1	50.0	57.4	58.3	2365 bp	1.0	49.1
ndhF_p5	GGATGGGAATCAAGAAAATTCGA	GAGGTAAGATCCGGCGAATC	39.1	55.0	57.2	57.9	2363 bp	1.0	48.8