Hotspot

clpP

LSC · 71794-73842 · 2049 bp

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Pi 0.000651
MarkerSeek score 58.10
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2049
variable_sites
2
pi
0.000651
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 GGGCTGGTTTAGATCGATCC ACCCATTGTTACGTTTCCACA 55.0 42.9 57.8 58.1 2230-2231 bp 1.0 70.2
clpP_p2 TCGCGGATTTGCATGAAATC ACCCATTGTTACGTTTCCACA 45.0 42.9 57.5 58.1 2141-2142 bp 1.0 67.8
clpP_p3 GGGCTGGTTTAGATCGATCC CCCATTGTTACGTTTCCACA 55.0 45.0 57.8 56.3 2229-2230 bp 1.0 64.8
clpP_p4 GGGCTGGTTTAGATCGATCC ACCCATTGTTACGTTTCCAC 55.0 45.0 57.8 56.3 2230-2231 bp 1.0 64.8
clpP_p5 TGGGCTGGTTTAGATCGATC ACCCATTGTTACGTTTCCACA 50.0 42.9 57.1 58.1 2231-2232 bp 1.0 64.3