Hotspot

ycf1-trnN-GUU

IRa · 131588-131909 · 322 bp

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Pi 0
MarkerSeek score 26.00
Primer available yes
Label ycf1-trnN-GUU

Feature Metadata

label_name
ycf1-trnN-GUU
feature_type
igs
region
IRa
length_bp
322
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
26.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-trnN-GUU_p1 ATAGAGTCCGACCACAACGA ACGACCAGTCAGTTAACAGC 50.0 50.0 58.2 57.8 455 bp 1.0 77.4
ycf1-trnN-GUU_p2 ATAGAGTCCGACCACAACGA AGGATTCGAACCTACGACCA 50.0 50.0 58.2 58.2 468 bp 1.0 77.4
ycf1-trnN-GUU_p3 ACCACAACGACCGAATTGAT ACGACCAGTCAGTTAACAGC 45.0 50.0 57.8 57.8 445 bp 1.0 77.4
ycf1-trnN-GUU_p4 ACCACAACGACCGAATTGAT AGGATTCGAACCTACGACCA 45.0 50.0 57.8 58.2 458 bp 1.0 77.3
ycf1-trnN-GUU_p5 ATAGAGTCCGACCACAACGA GGATTCGAACCTACGACCAG 50.0 55.0 58.2 57.8 467 bp 1.0 77.0