Hotspot

psbE-petL

LSC · 66345-67633 · 1289 bp

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Pi 0.00155
MarkerSeek score 59.70
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1289
variable_sites
3
pi
0.00155
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GTCGTATCTTGCTCAGACCA 50.0 50.0 57.9 57.1 1481 bp 1.0 72.2
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC GTCGTATCTTGCTCAGACCA 50.0 50.0 57.8 57.1 1404 bp 1.0 71.4
psbE-petL_p3 ACGAATAACCAACCCGCAAT GTCGTATCTTGCTCAGACCA 45.0 50.0 58.2 57.1 1490 bp 1.0 71.3
psbE-petL_p4 TGCTGACGAATAACCAACCC GTCGTATCTTGCTCAGACCA 50.0 50.0 58.2 57.1 1495 bp 1.0 71.2
psbE-petL_p5 CGAATAACCAACCCGCAATG GTCGTATCTTGCTCAGACCA 50.0 50.0 57.8 57.1 1489 bp 1.0 71.1