Hotspot

trnF-GAA-ndhJ

LSC · 49875-50550 · 676 bp

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Pi 0.00197
MarkerSeek score 47.50
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
676
variable_sites
2
pi
0.00197
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA AGTTGGATAGGATGGCCCTT 55.0 50.0 58.0 58.1 818 bp 1.0 79.5
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA AGTTGGATAGGATGGCCCTT 50.0 50.0 58.1 58.1 782 bp 1.0 79.1
trnF-GAA-ndhJ_p3 ATGATGCATCGGGAATGGTC AGTTGGATAGGATGGCCCTT 50.0 50.0 58.1 58.1 838 bp 1.0 78.7
trnF-GAA-ndhJ_p4 GATGATGCATCGGGAATGGT AGTTGGATAGGATGGCCCTT 50.0 50.0 58.1 58.1 839 bp 1.0 78.7
trnF-GAA-ndhJ_p5 GGGATAGCTCAGCTGGTAGA ATCATCCACGCTTGAAACGT 55.0 45.0 58.0 58.2 853 bp 1.0 78.6