Hotspot

ycf3-trnS-GGA

LSC · 45653-46518 · 866 bp

Back to Nyssa

Pi 0.00154
MarkerSeek score 59.70
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
866
variable_sites
2
pi
0.00154
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CCGTTTATCCGCGATCTAGG CCCTCGGTAAACAAAAGCCT 55.0 50.0 58.0 58.1 956 bp 1.0 79.2
ycf3-trnS-GGA_p2 CCGTTTATCCGCGATCTAGG ACGGAAAGAGAGGGATTCGA 55.0 50.0 58.0 58.2 977 bp 1.0 78.8
ycf3-trnS-GGA_p3 CCGTTTATCCGCGATCTAGG TTCCAATGCTACGCCTTGAA 55.0 45.0 58.0 57.8 927 bp 1.0 78.5
ycf3-trnS-GGA_p4 CCGTTTATCCGCGATCTAGG ACCCTCGGTAAACAAAAGCC 55.0 50.0 58.0 58.4 957 bp 1.0 76.9
ycf3-trnS-GGA_p5 GTTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.6 58.1 954 bp 1.0 75.9