Hotspot

rpoB-trnC-GCA

LSC · 27293-28466 · 1174 bp

Back to Nyssa

Pi 0.00114
MarkerSeek score 59.50
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1174
variable_sites
2
pi
0.00114
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 GTTCTTCCGTCAAACCCTGA CCGGATTTGAACTGGGGAAA 50.0 50.0 57.8 58.1 1339-1340 bp 1.0 77.5
rpoB-trnC-GCA_p2 AGTTCTTCCGTCAAACCCTG CCGGATTTGAACTGGGGAAA 50.0 50.0 57.7 58.1 1340-1341 bp 1.0 77.4
rpoB-trnC-GCA_p3 CTTCCGTCAAACCCTGATCA CCGGATTTGAACTGGGGAAA 50.0 50.0 57.5 58.1 1336-1337 bp 1.0 75.7
rpoB-trnC-GCA_p4 GTTCTTCCGTCAAACCCTGA AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.8 58.4 1321-1322 bp 1.0 75.3
rpoB-trnC-GCA_p5 AGTTCTTCCGTCAAACCCTG AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.7 58.4 1322-1323 bp 1.0 75.2