Hotspot

rps2-rpoC2

LSC · 16712-16929 · 218 bp

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Pi 0.00612
MarkerSeek score 56.90
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
218
variable_sites
2
pi
0.00612
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 AACTCCTGCCTCCATCATCT TTTTGTTCCACCACCGAAAA 50.0 40.0 58.1 56.3 366-367 bp 1.0 66.1
rps2-rpoC2_p2 TGCCTCCATCATCTCTTCCA TTTTGTTCCACCACCGAAAA 50.0 40.0 58.1 56.3 360-361 bp 1.0 66.0
rps2-rpoC2_p3 TCCTGCCTCCATCATCTCTT TTTTGTTCCACCACCGAAAA 50.0 40.0 57.8 56.3 363-364 bp 1.0 65.5
rps2-rpoC2_p4 GAACTCCTGCCTCCATCATC TTTTGTTCCACCACCGAAAA 55.0 40.0 57.7 56.3 367-368 bp 1.0 64.9
rps2-rpoC2_p5 CTCCTGCCTCCATCATCTCT TTTTGTTCCACCACCGAAAA 55.0 40.0 58.3 56.3 364-365 bp 1.0 64.7