Hotspot

ycf1

SSC · 122728-128205 · 5478 bp

Back to Nymphoides

Pi 0.0159

MarkerSeek score 51.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5478
variable_sites: 211
pi: 0.0159
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGTTTTACGCCTTTGAGCAC	TGCCGAAGTGATGGAAAACA	50.0	45.0	58.0	58.0	855-873 bp	1.0	84.8
ycf1_p2	GGTTTTACGCCTTTGAGCAC	CCCAGTTTGGCAACTTTTGG	50.0	50.0	58.0	58.0	813-831 bp	1.0	84.8
ycf1_p3	GGTTTTACGCCTTTGAGCAC	AAACTCCCCTGAGATGGTCA	50.0	50.0	58.0	58.0	263-272 bp	0.833333	78.9
ycf1_p4	TTTGAGCACGCATAGATCCC	TGCCGAAGTGATGGAAAACA	50.0	45.0	58.0	58.0	844 bp	0.166667	46.1
ycf1_p5	TTTGAGCACGCATAGATCCC	AAACTCCCCTGAGATGGTCA	50.0	50.0	58.0	58.0	252 bp	0.166667	45.9