Hotspot

rpl22

LSC · 83374-83865 · 492 bp

Back to Nymphoides

Pi 0.0419

MarkerSeek score 79.20

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 492
variable_sites: 45
pi: 0.0419
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	AGGGAATGATGACCTTGGGT	TCATTGATTAGTAGGAGACGAACT	50.0	37.5	58.3	57.0	546-600 bp	1.0	48.9
rpl22_p2	GGGAATGATGACCTTGGGTT	TCATTGATTAGTAGGAGACGAACT	50.0	37.5	57.5	57.0	545-599 bp	1.0	48.9
rpl22_p3	AGGGAATGATGACCTTGGGT	TCATTGATTAGTAGGAGACGAAC	50.0	39.1	58.3	55.6	546-600 bp	1.0	48.9
rpl22_p4	AGGGAATGATGACCTTGGGT	CATTGATTAGTAGGAGACGAACT	50.0	39.1	58.3	55.6	545-599 bp	1.0	48.9
rpl22_p5	GGGCAAACCAAAGGGAATGA	TCATTGATTAGTAGGAGACGAACT	50.0	37.5	58.7	57.0	557-611 bp	1.0	48.8