Hotspot

ndhF-rpl32

SSC · 111504-112207 · 704 bp

Back to Nymphoides

Pi 0.0327

MarkerSeek score 73.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 704
variable_sites: 46
pi: 0.0327
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AACGCTCATAAAAGCCCACA	TTAACGCTGCCCAATATCCC	45.0	50.0	58.1	58.0	899-941 bp	1.0	85.7
ndhF-rpl32_p2	AGACTCTTTGTTGCTGTCGG	TTAACGCTGCCCAATATCCC	50.0	50.0	58.1	58.0	871-913 bp	1.0	85.6
ndhF-rpl32_p3	ACGCTCATAAAAGCCCACAT	TTAACGCTGCCCAATATCCC	45.0	50.0	57.9	58.0	898-940 bp	1.0	85.4
ndhF-rpl32_p4	AAGGGGAAATTGTGCCGATT	TTAACGCTGCCCAATATCCC	45.0	50.0	58.1	58.0	1502-1544 bp	1.0	83.8
ndhF-rpl32_p5	AGGGGAAATTGTGCCGATTT	TTAACGCTGCCCAATATCCC	45.0	50.0	58.1	58.0	1501-1543 bp	1.0	83.8