Hotspot

rpl16-rps3

LSC · 81478-82732 · 1255 bp

Back to Nymphoides

Pi 0.0166

MarkerSeek score 66.60

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1255
variable_sites: 50
pi: 0.0166
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CGGAATCTTGTTCTTTTGGGG	TGTTCCTATACAGTGAGAACCA	47.6	40.9	57.4	56.2	1360-1395 bp	1.0	46.0
rpl16-rps3_p2	CGGAATCTTGTTCTTTTGGGG	TTGTTCCTATACAGTGAGAACCA	47.6	39.1	57.4	56.9	1361-1396 bp	1.0	46.0
rpl16-rps3_p3	CGGAATCTTGTTCTTTTGGGG	TGTTCCTATACAGTGAGAACCATC	47.6	41.7	57.4	57.8	1360-1395 bp	1.0	46.0
rpl16-rps3_p4	CGGAATCTTGTTCTTTTGGGG	TGTTCCTATACAGTGAGAACCAT	47.6	39.1	57.4	56.7	1360-1395 bp	1.0	46.0
rpl16-rps3_p5	CGGAATCTTGTTCTTTTGGGG	GTTCCTATACAGTGAGAACCATCT	47.6	41.7	57.4	57.5	1359-1394 bp	1.0	46.0