Hotspot

trnT-UGU-trnL-UAA

LSC · 46772-47347 · 576 bp

Back to Nymphoides

Pi 0.0193

MarkerSeek score 64.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 576
variable_sites: 27
pi: 0.0193
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CTTCCATCGAGTCTCTGCAC	55.0	55.0	57.9	58.1	791-816 bp	1.0	85.2
trnT-UGU-trnL-UAA_p2	AAAACCTGACCTGTTGACCC	CTTCCATCGAGTCTCTGCAC	50.0	55.0	57.9	58.1	1460-1497 bp	1.0	85.0
trnT-UGU-trnL-UAA_p3	AAAACCTGACCTGTTGACCC	AACGTTTCCGGTCAGATCAG	50.0	50.0	57.9	57.9	1976-2033 bp	1.0	84.9
trnT-UGU-trnL-UAA_p4	AAAACCTGACCTGTTGACCC	CGTTTCCGGTCAGATCAGTT	50.0	50.0	57.9	57.9	1974-2031 bp	1.0	84.9
trnT-UGU-trnL-UAA_p5	AAAACCTGACCTGTTGACCC	CGTACCAAGGCCCAATACAA	50.0	50.0	57.9	57.9	1313-1350 bp	1.0	84.9