Hotspot

rpoB-trnC-GCA

LSC · 27058-28356 · 1299 bp

Back to Nymphoides

Pi 0.0162

MarkerSeek score 66.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1299
variable_sites: 49
pi: 0.0162
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGTTCTTCTGTCAAACCCTGA	CCGGATTTGAACTGGGGAAA	42.9	50.0	56.8	58.1	1396-1450 bp	1.0	68.4
rpoB-trnC-GCA_p2	TCAAACCCTGATCAATGAACCT	CCGGATTTGAACTGGGGAAA	40.9	50.0	57.6	58.1	1386-1440 bp	1.0	67.0
rpoB-trnC-GCA_p3	TCTTCTGTCAAACCCTGATCA	CCGGATTTGAACTGGGGAAA	42.9	50.0	56.6	58.1	1393-1447 bp	1.0	66.7
rpoB-trnC-GCA_p4	GTCAAACCCTGATCAATGAACC	CCGGATTTGAACTGGGGAAA	45.5	50.0	57.6	58.1	1387-1441 bp	1.0	66.6
rpoB-trnC-GCA_p5	AGTTCTTCTGTCAAACCCTGA	AAAAGGATTTGCAGTCCCCC	42.9	50.0	56.8	58.4	1378-1432 bp	1.0	66.2