Hotspot

atpA-atpF

LSC · 11809-11876 · 68 bp

Back to Nymphoides

Pi 0.0456

MarkerSeek score 75.10

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 68
variable_sites: 6
pi: 0.0456
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	TTTCGTCTGCTCGAATGGTT	ATTGGGGGCGATGAAAGAAA	45.0	45.0	57.8	57.8	123-135 bp	1.0	82.0
atpA-atpF_p2	TTTCGTCTGCTCGAATGGTT	TTGGGGGCGATGAAAGAAAT	45.0	45.0	57.8	57.8	122-134 bp	1.0	82.0
atpA-atpF_p3	TCGTCTGCTCGAATGGTTAC	ATTGGGGGCGATGAAAGAAA	50.0	45.0	57.7	57.8	121-133 bp	1.0	81.2
atpA-atpF_p4	TCGTCTGCTCGAATGGTTAC	TTGGGGGCGATGAAAGAAAT	50.0	45.0	57.7	57.8	120-132 bp	1.0	81.2
atpA-atpF_p5	GTCTGCTCGAATGGTTACCA	TTGGGGGCGATGAAAGAAAT	50.0	45.0	57.6	57.8	118-130 bp	1.0	80.4