Hotspot

trnH-GUG-psbA

LSC · 98-580 · 483 bp

Back to Nymphoides

Pi 0.0423

MarkerSeek score 78.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 483
variable_sites: 44
pi: 0.0423
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCGAAGCTCCATCTACAAATGG	55.0	45.5	58.1	58.2	508-539 bp	1.0	72.1
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TCGAAGCTCCATCTACAAATGG	50.0	45.5	57.9	58.2	522-553 bp	1.0	71.9
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TCGAAGCTCCATCTACAAATGG	50.0	45.5	57.9	58.2	521-552 bp	1.0	71.9
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	CGAAGCTCCATCTACAAATGG	55.0	47.6	58.1	56.8	507-538 bp	1.0	71.9
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	TCGAAGCTCCATCTACAAATGG	50.0	45.5	57.9	58.2	523-554 bp	1.0	71.8