Hotspot

ndhF

SSC · 115236-117479 · 2244 bp

Back to Nymphaea

Pi 0.0103

MarkerSeek score 45.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 105
pi: 0.0103
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AATTCGGTCGTTATGGTCGG	TTAAAGCATAGCGTCCCCAC	50.0	50.0	58.1	58.0	2912-2979 bp	0.83871	79.2
ndhF_p2	GAGCTACTGAGGAACAACGG	AGTCGAGGAAGAGCGAGATT	55.0	50.0	58.0	58.2	2976-2999 bp	0.451613	60.6
ndhF_p3	AATTCGGTCGTTATGGTCGG	ATAGCGTCCCCACGATTAGA	50.0	50.0	58.1	58.0	2923-2936 bp	0.193548	48.9
ndhF_p4	AATTCGGTCGTTATGGTCGG	CATAGCGTCCCCACGATTAG	50.0	55.0	58.1	57.9	2924-2937 bp	0.193548	48.4
ndhF_p5	AATTCGGTCGTTATGGTCGG	TAGCGTCCCCACGATTAGAA	50.0	50.0	58.1	58.2	2922-2935 bp	0.193548	47.1