Hotspot

ccsA-ndhD

SSC · 120325-120689 · 365 bp

Back to Nymphaea

Pi 0.0315

MarkerSeek score 64.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 365
variable_sites: 35
pi: 0.0315
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCAATTGTGGCTTCTATGGG	TCCAGATTTCGTTCTTTCACT	50.0	38.1	57.1	55.2	522-582 bp	1.0	52.2
ccsA-ndhD_p2	GCAATTGTGGCTTCTATGGG	CGTTCTTTCACTATCAGTTGACA	50.0	39.1	57.1	56.8	513-573 bp	1.0	49.9
ccsA-ndhD_p3	GCAATTGTGGCTTCTATGGG	TCGTTCTTTCACTATCAGTTGACA	50.0	37.5	57.1	58.2	514-574 bp	1.0	49.9
ccsA-ndhD_p4	GCAAATTCAGCAATTGTGGC	TCCAGATTTCGTTCTTTCACT	45.0	38.1	57.1	55.2	531-591 bp	0.870968	46.4
ccsA-ndhD_p5	GCAAATTCAGCAATTGTGGC	CGTTCTTTCACTATCAGTTGACA	45.0	39.1	57.1	56.8	522-582 bp	0.870968	44.2