Hotspot

petD-rpoA

LSC · 83420-83843 · 424 bp

Back to Nymphaea

Pi 0.0363

MarkerSeek score 66.20

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 424
variable_sites: 62
pi: 0.0363
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GCCCAGTAGCTACAACAGTC	TGCTGTATCCATGCCTGTTC	55.0	50.0	58.0	58.0	1051-1098 bp	1.0	88.5
petD-rpoA_p2	GTTCTTTCGGGGGATGGATC	TGCTGTATCCATGCCTGTTC	55.0	50.0	58.0	58.0	1462-1509 bp	1.0	86.0
petD-rpoA_p3	GCTCGAGCTGGATGATGAAA	TGCTGTATCCATGCCTGTTC	50.0	50.0	58.1	58.0	1496-1543 bp	1.0	85.7
petD-rpoA_p4	GCCCAGTAGCTACAACAGTC	CAGAACCGGTTGATTTGTGC	55.0	50.0	58.0	57.9	1151-1198 bp	0.645161	70.7
petD-rpoA_p5	GTTCTTTCGGGGGATGGATC	CAGAACCGGTTGATTTGTGC	55.0	50.0	58.0	57.9	1562-1609 bp	0.645161	69.1