Hotspot

petA-psbJ

LSC · 68257-69479 · 1223 bp

Back to Nymphaea

Pi 0.0202
MarkerSeek score 62.50
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1223
variable_sites
94
pi
0.0202
unique_haplotype_count
24
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGAGAAGGTTCAATTAGCCGA ATTCCGGATTGGGATCATCC 42.9 50.0 57.0 57.1 1255-1311 bp 0.967742 65.6
petA-psbJ_p2 TGAGAAGGTTCAATTAGCCGAA ATTCCGGATTGGGATCATCC 40.9 50.0 57.7 57.1 1255-1311 bp 0.967742 63.2
petA-psbJ_p3 TTGAGAAGGTTCAATTAGCCGA ATTCCGGATTGGGATCATCC 40.9 50.0 57.7 57.1 1256-1312 bp 0.967742 63.2
petA-psbJ_p4 TGAGAAGGTTCAATTAGCCG ATTCCGGATTGGGATCATCC 45.0 50.0 55.5 57.1 1255-1311 bp 0.967742 61.1
petA-psbJ_p5 TTGAGAAGGTTCAATTAGCCG ATTCCGGATTGGGATCATCC 42.9 50.0 56.3 57.1 1256-1312 bp 0.967742 59.4