Hotspot

tRNA-Leu (UAA)-tRNA-Phe (GAA)

LSC · 51415-51795 · 381 bp

Back to Nymphaea

Pi 0.0268

MarkerSeek score 66.20

Primer available yes

Label tRNA-Leu (UAA)-tRNA-Phe (GAA)

Feature Metadata

label_name: tRNA-Leu (UAA)-tRNA-Phe (GAA)
feature_type: igs
region: LSC
length_bp: 381
variable_sites: 36
pi: 0.0268
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Leu (UAA)-tRNA-Phe (GAA)_p1	AAATCGTGAGGGTTCGAGTC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.6	58.0	435-464 bp	1.0	86.0
tRNA-Leu (UAA)-tRNA-Phe (GAA)_p2	GAAATCGTGAGGGTTCGAGT	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.6	58.0	436-465 bp	1.0	85.9
tRNA-Leu (UAA)-tRNA-Phe (GAA)_p3	GGAGGAAAATCCGTCGACTT	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.6	58.0	458-487 bp	1.0	85.4
tRNA-Leu (UAA)-tRNA-Phe (GAA)_p4	AAATCGTGAGGGTTCGAGTC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.6	58.1	471-500 bp	1.0	84.9
tRNA-Leu (UAA)-tRNA-Phe (GAA)_p5	GAAATCGTGAGGGTTCGAGT	TTTGAACTGGTGACACGAGG	50.0	50.0	57.6	58.1	472-501 bp	1.0	84.9