Hotspot

rpoB-tRNA-Cys (GCA)

LSC · 28864-30169 · 1306 bp

Back to Nymphaea

Pi 0.0164

MarkerSeek score 65.70

Primer available yes

Label rpoB-tRNA-Cys (GCA)

Feature Metadata

label_name: rpoB-tRNA-Cys (GCA)
feature_type: igs
region: LSC
length_bp: 1306
variable_sites: 84
pi: 0.0164
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-tRNA-Cys (GCA)_p1	ACATTCCCTCATCTCCACCA	TTGTTGTGTTGATCAGGCGA	50.0	45.0	58.0	58.0	1337-1432 bp	0.935484	85.8
rpoB-tRNA-Cys (GCA)_p2	ACATTCCCTCATCTCCACCA	CTGGGGAGAAAGGATTTGCA	50.0	50.0	58.0	57.8	1303-1398 bp	0.935484	84.4
rpoB-tRNA-Cys (GCA)_p3	ACATTCCCTCATCTCCACCA	TGGGGAGAAAGGATTTGCAG	50.0	50.0	58.0	57.8	1302-1397 bp	0.935484	84.4
rpoB-tRNA-Cys (GCA)_p4	ACATTCCCTCATCTCCACCA	AGAAAGGATTTGCAGTCCCC	50.0	50.0	58.0	57.8	1297-1392 bp	0.935484	84.4
rpoB-tRNA-Cys (GCA)_p5	ACATTCCCTCATCTCCACCA	GGGGAGAAAGGATTTGCAGT	50.0	50.0	58.0	57.8	1301-1396 bp	0.935484	84.4