Hotspot

atpF-atpH

LSC · 14682-15063 · 382 bp

Back to Nymphaea

Pi 0.154

MarkerSeek score 69.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 382
variable_sites: 144
pi: 0.154
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TAAAGTGCAGCTGGTTGGTT	AATTTACGGACTGGTCGTGG	45.0	50.0	57.9	57.9	623-677 bp	1.0	90.8
atpF-atpH_p2	GATCTCTGGAGGGCTCTTCT	AATTTACGGACTGGTCGTGG	55.0	50.0	57.9	57.9	1111-1159 bp	1.0	90.6
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	AATTTACGGACTGGTCGTGG	50.0	50.0	57.9	57.9	496-550 bp	1.0	90.3
atpF-atpH_p4	TACACAACTCGCACACACTC	AATTTACGGACTGGTCGTGG	50.0	50.0	58.2	57.9	586-640 bp	1.0	90.1
atpF-atpH_p5	GCCTACCCTCTCTTCTCTGT	AATTTACGGACTGGTCGTGG	55.0	50.0	57.8	57.9	1169-1219 bp	1.0	90.1