Hotspot

tRNA-Lys (UUU)-rps16

LSC · 4497-5299 · 803 bp

Back to Nymphaea

Pi 0.0144

MarkerSeek score 67.90

Primer available yes

Label tRNA-Lys (UUU)-rps16

Feature Metadata

label_name: tRNA-Lys (UUU)-rps16
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 56
pi: 0.0144
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Lys (UUU)-rps16_p1	CCGATGGTATGGACGAATCC	AAAAAGGAGCTCAACCCACG	55.0	50.0	57.9	58.7	969-1060 bp	0.967742	81.4
tRNA-Lys (UUU)-rps16_p2	ACGAATCCGCTTCATCCAAA	AAAAAGGAGCTCAACCCACG	45.0	50.0	57.9	58.7	957-1048 bp	0.967742	81.3
tRNA-Lys (UUU)-rps16_p3	CCGATGGTATGGACGAATCC	TGAAAAAGGAGCTCAACCCA	55.0	45.0	57.9	57.3	971-1062 bp	0.967742	80.9
tRNA-Lys (UUU)-rps16_p4	AAAGCCGAGTACTCTACCGT	AAAAAGGAGCTCAACCCACG	50.0	50.0	58.2	58.7	911-1002 bp	0.967742	80.9
tRNA-Lys (UUU)-rps16_p5	AAGCCGAGTACTCTACCGTT	AAAAAGGAGCTCAACCCACG	50.0	50.0	58.2	58.7	910-1001 bp	0.967742	80.9