Hotspot

ndhF-rpl32

SSC · 118426-119536 · 1111 bp

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Pi 0.0116
MarkerSeek score 59.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1111
variable_sites
22
pi
0.0116
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGGAATAAGCAGGAGTCCCA GCTGCCCAATATCCCTTTCT 50.0 50.0 58.0 57.6 1266-1284 bp 1.0 79.6
ndhF-rpl32_p2 AAGCAGGAGTCCCAATCCTA GCTGCCCAATATCCCTTTCT 50.0 50.0 57.7 57.6 1260-1278 bp 1.0 77.7
ndhF-rpl32_p3 AGCAGGAGTCCCAATCCTAT GCTGCCCAATATCCCTTTCT 50.0 50.0 57.5 57.6 1259-1277 bp 1.0 75.8
ndhF-rpl32_p4 TGCTGTTGGAATAAGCAGGA GCTGCCCAATATCCCTTTCT 45.0 50.0 57.1 57.6 1272-1290 bp 1.0 72.8
ndhF-rpl32_p5 GGAATAAGCAGGAGTCCCAA GCTGCCCAATATCCCTTTCT 50.0 50.0 56.9 57.6 1265-1283 bp 1.0 71.0