Hotspot

rps19-trnH-GUG

IRb · 90331-90518 · 188 bp

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Pi 0.0641
MarkerSeek score 60.70
Primer available yes
Label rps19-trnH-GUG

Feature Metadata

label_name
rps19-trnH-GUG
feature_type
igs
region
IRb
length_bp
188
variable_sites
6
pi
0.0641
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.28
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-trnH-GUG_p1 CTCCACGTTGAGCTTCTCAA GAATGATGTGGATGGGCGAA 50.0 50.0 57.8 58.3 145-352 bp 1.0 82.6
rps19-trnH-GUG_p2 CTCCACGTTGAGCTTCTCAA ATGAATGATGTGGATGGGCG 50.0 50.0 57.8 58.4 147-354 bp 1.0 82.1
rps19-trnH-GUG_p3 CTCCACGTTGAGCTTCTCAA AATGATGTGGATGGGCGAAC 50.0 50.0 57.8 58.6 144-351 bp 0.75 70.1
rps19-trnH-GUG_p4 CTCCACGTTGAGCTTCTCAA AATCCACTTGGCTACATCCG 50.0 50.0 57.8 57.7 285 bp 0.25 46.1
rps19-trnH-GUG_p5 CTCCACGTTGAGCTTCTCAA AACGACGGGAATTGAACCC 50.0 52.6 57.8 58.1 334 bp 0.25 40.3