Hotspot

petD-rpoA

LSC · 83789-84187 · 399 bp

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Pi 0.0343
MarkerSeek score 72.10
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
399
variable_sites
26
pi
0.0343
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 AGTTGATCCTCCCTAGGTACA GGGTTTCACAATCGACTTGC 47.6 50.0 56.9 57.7 474-486 bp 1.0 68.0
petD-rpoA_p2 TCAAAGTTGATCCTCCCTAGGT GGGTTTCACAATCGACTTGC 45.5 50.0 58.2 57.7 478-490 bp 1.0 66.9
petD-rpoA_p3 CAAAGTTGATCCTCCCTAGGT GGGTTTCACAATCGACTTGC 47.6 50.0 56.7 57.7 477-489 bp 1.0 66.2
petD-rpoA_p4 AAGTTGATCCTCCCTAGGTACA GGGTTTCACAATCGACTTGC 45.5 50.0 57.7 57.7 475-487 bp 1.0 65.7
petD-rpoA_p5 TGATCCTCCCTAGGTACATCTC GGGTTTCACAATCGACTTGC 50.0 50.0 57.6 57.7 471-483 bp 1.0 65.5