Hotspot

psbE-petL

LSC · 70680-71963 · 1284 bp

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Pi 0.00701
MarkerSeek score 58.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1284
variable_sites
17
pi
0.00701
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 ACGAATGACCAACCTGCAAT GTCGTATCTTGCTCAGACCA 45.0 50.0 58.1 57.1 1485-1543 bp 1.0 73.0
psbE-petL_p2 ACGAATGACCAACCTGCAAT GCTGGGGTTATAGTTGAAGCA 45.0 47.6 58.1 58.0 1456-1514 bp 1.0 72.6
psbE-petL_p3 ACGAATGACCAACCTGCAAT ACCAATGAATAGAGCTGGGG 45.0 50.0 58.1 57.0 1469-1527 bp 1.0 72.3
psbE-petL_p4 ACGAATGACCAACCTGCAAT CCAATGAATAGAGCTGGGGT 45.0 50.0 58.1 57.0 1468-1526 bp 1.0 72.3
psbE-petL_p5 CGAATGACCAACCTGCAATG GTCGTATCTTGCTCAGACCA 50.0 50.0 57.7 57.1 1484-1542 bp 1.0 71.6