Hotspot

ycf3-trnS-GGA

LSC · 48230-49121 · 892 bp

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Pi 0.0028
MarkerSeek score 60.70
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
892
variable_sites
5
pi
0.0028
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGCCTCTTTTTCTCCCGAAG TGCTACGCCTTAAACCACTC 50.0 50.0 57.8 57.9 1029-1042 bp 1.0 78.2
ycf3-trnS-GGA_p2 GCCTCTTTTTCTCCCGAAGT TGCTACGCCTTAAACCACTC 50.0 50.0 57.8 57.9 1028-1041 bp 1.0 78.2
ycf3-trnS-GGA_p3 TGCCTCTTTTTCTCCCGAAG CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.8 58.1 1064-1077 bp 1.0 78.1
ycf3-trnS-GGA_p4 GCCTCTTTTTCTCCCGAAGT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.8 58.1 1063-1076 bp 1.0 78.1
ycf3-trnS-GGA_p5 TGCCTCTTTTTCTCCCGAAG ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.8 58.2 1085-1098 bp 1.0 77.7