Hotspot

trnE-UUC-trnT-GGU

LSC · 34120-34914 · 795 bp

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Pi 0.00998
MarkerSeek score 59.80
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
795
variable_sites
11
pi
0.00998
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.91
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGATGTCCTGAACCGCTA ACCTTTGCAGCTTCATGGAA 50.0 45.0 57.9 58.0 834-920 bp 1.0 83.8
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA ACCTTTGCAGCTTCATGGAA 50.0 45.0 57.9 58.0 832-918 bp 1.0 83.8
trnE-UUC-trnT-GGU_p3 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 796-882 bp 1.0 83.8
trnE-UUC-trnT-GGU_p4 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 794-880 bp 1.0 83.8
trnE-UUC-trnT-GGU_p5 GATGTCCTGAACCGCTAGAC ACCTTTGCAGCTTCATGGAA 55.0 45.0 57.8 58.0 831-917 bp 1.0 83.3